Cloning Tags
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- MAGIC, an in
vivo genetic
method for the
rapid
construction
of recombinant
DNA molecules: Nature
Genetics, Vol.
37, No. 3. (30
January 2005),
pp. 311-319.
Source: Nature Genetics, Vol. 37, No. 3. (30 January 2005), pp. 311-319. - PCR-suppressio
n effect:
Kinetic
analysis and
application to
representative
or
long-molecule
biased
PCR-based
amplification
of complex
samples: Journal of
Biotechnology,
Vol. In Press,
Corrected
ProofIn the
present study,
we analyzed
the kinetics
of the
PCR-suppressio
n effect
(PS-effect)
and firstly
demonstrate
that lower
annealing
temperature
enhances
PS-effect.
Furthermore,
we controlled
the average
size of
complex PCR
products over
a wide range,
and
simultaneously
amplified
targets from
0.25 to 10 kb
by regulating
the degree of
suppression in
a
single-primer
PCR. In
addition, we
describe an
improved
template-switc
hing
full-length
cDNA synthesis
method that
greatly
reduces
truncated
cDNA. This
study provides
a general
guide for the
design of
PS-effect
related PCR
and is useful
for
representative
or
long-transcrip
t enriched
cDNA library
construction,
especially
when only a
small amount
of total RNA
is available.
Source: Journal of Biotechnology, Vol. In Press, Corrected Proof - Harnessing
homologous
recombination
in vitro to
generate
recombinant
DNA via SLIC: Nature
Methods, Vol.
4, No. 3. (11
February
2007), pp.
251-256.
Source: Nature Methods, Vol. 4, No. 3. (11 February 2007), pp. 251-256. - Generation and
Characterizati
on of SCARs by
Cloning and
Sequencing of
RAPD Products:
A Strategy for
Species-specif
ic Marker
Development in
Bamboo: Annals of
Botany, Vol.
95, No. 5. (16
April 2005),
pp. 835-841.
Source: Annals of Botany, Vol. 95, No. 5. (16 April 2005), pp. 835-841. - Structure of a
cannabinoid
receptor and
functional
expression of
the cloned
cDNA.: Nature, Vol.
346, No. 6284.
(9 August
1990), pp.
561-564.Mariju
ana and many
of its
constituent
cannabinoids
influence the
central
nervous system
(CNS) in a
complex and
dose-dependent
manner.
Although CNS
depression and
analgesia are
well
documented
effects of the
cannabinoids,
the mechanisms
responsible
for these and
other
cannabinoid-in
duced effects
are not so far
known. The
hydrophobic
nature of
these
substances has
suggested that
cannabinoids
resemble
anaesthetic
agents in
their action,
that is, they
nonspecificall
y disrupt
cellular
membranes.
Recent
evidence,
however, has
supported a
mechanism
involving a G
protein-couple
d receptor
found in brain
and neural
cell lines,
and which
inhibits
adenylate
cyclase
activity in a
dose-dependent
,
stereoselectiv
e and
pertussis
toxin-sensitiv
e manner.
Also, the
receptor is
more
responsive to
psychoactive
cannabinoids
than to
non-psychoacti
ve
cannabinoids.
Here we report
the cloning
and expression
of a
complementary
DNA that
encodes a G
protein-couple
d receptor
with all of
these
properties.
Its messenger
RNA is found
in cell lines
and regions of
the brain that
have
cannabinoid
receptors.
These findings
suggest that
this protein
is involved in
cannabinoid-in
duced CNS
effects
(including
alterations in
mood and
cognition)
experienced by
users of
marijuana.
Source: Nature, Vol. 346, No. 6284. (9 August 1990), pp. 561-564.
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